402 pcdna3 1 zeo expression vector Search Results


402 pcdna3 1 zeo expression vector  (Thermo Fisher)
99
Thermo Fisher 402 pcdna3 1 zeo expression vector
402 Pcdna3 1 Zeo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/402+pcdna3+1+zeo+expression+vector/Zeocin/ppr0789041-213-65-69
Average 99 stars, based on 1 article reviews
402 pcdna3 1 zeo expression vector - by Bioz Stars, 2026-11
99/100 stars
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pcdna3 1 hsrebp2 402 egfp  (Addgene inc)
96
Addgene inc pcdna3 1 hsrebp2 402 egfp
Pcdna3 1 Hsrebp2 402 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/402+pcdna3+1+zeo+expression+vector/pcDNA3-EGFP+(Plasmid+%2313031)/pm33974044-40-28-26
Average 96 stars, based on 1 article reviews
pcdna3 1 hsrebp2 402 egfp - by Bioz Stars, 2026-11
96/100 stars
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pcdna3 1 morange plasmid  (Addgene inc)
96
Addgene inc pcdna3 1 morange plasmid
Pcdna3 1 Morange Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/402+pcdna3+1+zeo+expression+vector/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/pmc04718004-462-28-5
Average 96 stars, based on 1 article reviews
pcdna3 1 morange plasmid - by Bioz Stars, 2026-11
96/100 stars
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mettl3  (Addgene inc)
90
Addgene inc mettl3
Fig. 1 | Targeted 5′ UTR methylation by engineered m6A ‘writers’ a, Schematic of engineered m6A ‘writers’ by coupling CRISPR-dCas9 with single-chain methyltransferase domains derived from <t>METTL3</t> (green) and METTL14 (brown). The sgRNA and PAMmer are base-paired to nearby regions of the m6A consensus motif on target RNA. PAMer supplies the PAM sequence NGG that does not need to be present on target RNA. b, Measurement of m6A levels on Hspa1a 5′ UTR-Luc in transfected HeLa cells. λ2 sgRNA was used as the non-targeting control. Primers were designed to span the targeting m6A site A103 in Hspa1a 5′ UTR or A1280 of Fluc mRNA as control for off-targeting. Error bars, mean ± s.e.m.; unpaired Student’s t-test, *P < 0.05, **P < 0.01; n = 4 independent experiments. c, Site-specific detection of m6A on Hspa1a 5′ UTR-Luc in transfected HeLa cells using fluorescein-dUTP-incorporated primer-extension approach. Both specific (A103) and non-specific (A1280) sites were detected from the same sample. Red boxes highlight decreased probe +1 signals as a result of increased methylation by M3M14-dCas9 but not the inactive mutant. This experiment was repeated twice with similar results. Uncropped scans are shown in Supplementary Fig. 11. d, Measurement of m6A levels on endogenous Hsph1 5′ UTR in MEF cells transfected with engineered m6A ‘writers’. λ2 sgRNA was used as the non-targeting control. Error bars, mean ± s.e.m.; unpaired Student’s t-test, *P < 0.05; n = 4 independent experiments.
Mettl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/402+pcdna3+1+zeo+expression+vector/NC3+pCDNA3%2E1+FLAG+WTX+(1-804)+(Plasmid+%2336958)/10__1038_slash_s41589___019___0327___1-258-6-26
Average 90 stars, based on 1 article reviews
mettl3 - by Bioz Stars, 2026-11
90/100 stars
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Image Search Results


Fig. 1 | Targeted 5′ UTR methylation by engineered m6A ‘writers’ a, Schematic of engineered m6A ‘writers’ by coupling CRISPR-dCas9 with single-chain methyltransferase domains derived from METTL3 (green) and METTL14 (brown). The sgRNA and PAMmer are base-paired to nearby regions of the m6A consensus motif on target RNA. PAMer supplies the PAM sequence NGG that does not need to be present on target RNA. b, Measurement of m6A levels on Hspa1a 5′ UTR-Luc in transfected HeLa cells. λ2 sgRNA was used as the non-targeting control. Primers were designed to span the targeting m6A site A103 in Hspa1a 5′ UTR or A1280 of Fluc mRNA as control for off-targeting. Error bars, mean ± s.e.m.; unpaired Student’s t-test, *P < 0.05, **P < 0.01; n = 4 independent experiments. c, Site-specific detection of m6A on Hspa1a 5′ UTR-Luc in transfected HeLa cells using fluorescein-dUTP-incorporated primer-extension approach. Both specific (A103) and non-specific (A1280) sites were detected from the same sample. Red boxes highlight decreased probe +1 signals as a result of increased methylation by M3M14-dCas9 but not the inactive mutant. This experiment was repeated twice with similar results. Uncropped scans are shown in Supplementary Fig. 11. d, Measurement of m6A levels on endogenous Hsph1 5′ UTR in MEF cells transfected with engineered m6A ‘writers’. λ2 sgRNA was used as the non-targeting control. Error bars, mean ± s.e.m.; unpaired Student’s t-test, *P < 0.05; n = 4 independent experiments.

Journal: Nature Chemical Biology

Article Title: Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates

doi: 10.1038/s41589-019-0327-1

Figure Lengend Snippet: Fig. 1 | Targeted 5′ UTR methylation by engineered m6A ‘writers’ a, Schematic of engineered m6A ‘writers’ by coupling CRISPR-dCas9 with single-chain methyltransferase domains derived from METTL3 (green) and METTL14 (brown). The sgRNA and PAMmer are base-paired to nearby regions of the m6A consensus motif on target RNA. PAMer supplies the PAM sequence NGG that does not need to be present on target RNA. b, Measurement of m6A levels on Hspa1a 5′ UTR-Luc in transfected HeLa cells. λ2 sgRNA was used as the non-targeting control. Primers were designed to span the targeting m6A site A103 in Hspa1a 5′ UTR or A1280 of Fluc mRNA as control for off-targeting. Error bars, mean ± s.e.m.; unpaired Student’s t-test, *P < 0.05, **P < 0.01; n = 4 independent experiments. c, Site-specific detection of m6A on Hspa1a 5′ UTR-Luc in transfected HeLa cells using fluorescein-dUTP-incorporated primer-extension approach. Both specific (A103) and non-specific (A1280) sites were detected from the same sample. Red boxes highlight decreased probe +1 signals as a result of increased methylation by M3M14-dCas9 but not the inactive mutant. This experiment was repeated twice with similar results. Uncropped scans are shown in Supplementary Fig. 11. d, Measurement of m6A levels on endogenous Hsph1 5′ UTR in MEF cells transfected with engineered m6A ‘writers’. λ2 sgRNA was used as the non-targeting control. Error bars, mean ± s.e.m.; unpaired Student’s t-test, *P < 0.05; n = 4 independent experiments.

Article Snippet: DNA sequences encoding methytransferase domain of METTL3 (residue 369–580) and METTL14 (residue 116–402) were amplified from pcDNA-Mettl3 and pcDNA-Mettl14, respectively, and subcloned into pCMV-BE2 vector backbone (Addgene no. 73020) using Gibson assembly (NEB E2611S).

Techniques: Methylation, CRISPR, Derivative Assay, Sequencing, Transfection, Control, Mutagenesis